Fig. 2
From: Cell shape-independent FtsZ dynamics in synthetically remodeled bacterial cells
Cell size independent recovery of fluorescence in FtsZ-rings. FRAP measurements on FtsZ-GFP rings in E. coli cells trapped standing in a vertical position. a Representative cells of different diameter, untreated or treated with drugs. White boxes indicate bleached areas. Scale bars = 1 μm. b Quantification of FRAP data from untreated cells (red), and drug-treated cells (other colors), showing that fluorescence recovery time is independent of cell diameter (in the range investigated, i.e., ~ 1–3 μm). ntot = 105. D = cell diameter range. Mean t1/2 recovery time WT FtsZ-rings was 8.4 ± 1.9 s (mean ± S.D., n = 23, red squares), while for large FtsZ-rings, recovery times were 8.2 ± 1.9 s (n = 7, dark blue circles), 8.4 ± 1.7 s (n = 27, light blue triangles), 8.3 ± 2.8 s (n = 30, green triangles) and 8.2 ± 2.6 s (n = 18, purple diamond) for cells with diameters in the ranges of 1–1.25 μm, 1.25–1.75 μm, 1.75–2.25 μm and > 2.25 μm, respectively. Error bars represent S.D. Note that FRAP measurements on fixed cells indicated that trapped cells were stationary, and not rotating in the holes (Supplementary Figure 10)
