Fig. 5
From: Cell shape-independent FtsZ dynamics in synthetically remodeled bacterial cells
FtsZ cluster dimensions and dynamics in heart-shaped cells. FtsZ behavior in E. coli cells sculpted into heart shapes. a Upper left, Cartoon representation of a WT E. coli cell and a heart shape (both colored red for visualization), highlighting the large and complex structural changes of a cell-to-heart transition, approximately to scale. Upper right, Drug-treated cell expressing cytoplasmic GFP, shaped as a heart. Lower, STED image of an FtsZ-heart (FtsZ-mNeonGreen) in a drug-treated E. coli cell. b Lengths and widths of 155 individual FtsZ-mNeonGreen fluorescence clusters in cells shaped as hearts. Average length = 129 ± 44 nm and width = 84 ± 9 nm. Boxes represent S.D., with red lines indicating mean. Whiskers on the box plots encompass 95.5% of the distribution. c Upper row, SIM image from a time-lapse series (epi-fluorescence) of a heart-shaped cell expressing FtsZ-mCitrine. Green arrowhead indicates internal FtsZ clustering. Corresponding kymograph is shown adjacent to the image, and was generated starting at the yellow arrowhead in the SIM image, moving counter-clockwise for the indicated length. The yellow arrow points to an FtsZ trajectory. Lower, average treadmilling speed of FtsZ-mCitrine (Z-mCit) clusters in hearts (22.6 ± 10.4 nm s−1, n = 44). d FRAP measurements of FtsZ-mCitrine in heart-shaped cells. Top row, bleaching of half the FtsZ-mCitrine molecules in a full heart. Bottom row, bleaching of a half-full heart. No difference in recovery times was observed. e Histogram of average t1/2 recovery times calculated from FRAP measurements. Recovery in full hearts: 7.1 ± 1.1 s (n = 24), recovery in half-hearts: 6.9 ± 0.9 s (n = 9). Scale bars = 1 μm. Dots represent individual data points, bars represent mean with error bars representing S.D
