Fig. 6
BAI3 and Stabilin-2 interact in cis in myoblasts to promote cell fusion. Stabilin-2 and BAI3 are required on the same myoblast to promote cell fusion. a Parental C2C12 cells were stained with membrane dyes and mixed in a 1:1 ratio. 24 h after plating, cells were switched into differentiation conditions for 48 h. b Parental C2C12 mixed with C2C12 overexpressing Flag-BAI3. c Parental C2C12 (green) mixed with C2C12 overexpressing V5-Stabilin-2 (purple). Cell fusion was intact and mixed fibers were observed after differentiation. d C2C12-expressing shRNA against BAI3 (green) are mixed with C2C12 overexpressing Stabilin-2 (purple). Cells without BAI3 were unable to fuse and mix with other population after differentiation. e C2C12 expressing shRNA against Stabilin-2 (purple) were mixed with C2C12 overexpressing BAI3 (green). Mixed fibers were not observed and only cells expressing both receptors were able to fuse together after differentiation. f Cells-expressing shRNA against Stabilin-2 or BAI3 were mixed and no fusion was observed following differentiation. The quantification of the phenotypes was analyzed from multiple images. Scale bar = 100 μm
