Fig. 5

ZNF207 co-localizes with enhancer markers to promote cell cycle of hESCs. a Top: Composite plot shows ZNF207, p300 (GSM1003513) and H3K27ac (GSM733718) ChIP-Seq signals are enriched and overlapped at TSSs. Bottom: Heatmap illustrates genome-wide association of ZNF207 with p300 and H3K27ac-binding sites. b Gene ontology analysis of genes that are co-bound by ZNF207, p300 and H3K27ac. Blue bars represent fold enrichment and orange bars represent p-value. p-value is calculated using hypergeometric distribution. c ChIP-Seq tracks show co-localization of ZNF207, P300, and H3K27ac at the regulatory sequences of cell cycle genes. The scale bars indicate the size of the chromosome. The light pink boxes highlight the co-bound regions. d Gene ontology analysis of cell cycle genes that are differentially expressed in ZNF207 KD cells. Blue bars represent p-values and orange bars represent FDR (false discovery rate). p-value is calculated using hypergeometric distribution. e Cell cycle progressions determined by flow cytometry. Histogram plot of flow cytometry analysis of control cells (red) and ZNF207 KD cells (blue). The percentage of cells that are in each phase of mitosis is shown. f ChIP-Seq tracks show binding of ZNF207 at promoter and enhancer regions of FGF2 gene. g The expression of FGF2 in control and ZNF207 KD cells. h Western blot to detect the protein level of FGF2 in KD, control, and OE cells. GAPDH is shown as the loading control. i Clonogenic assay to show the self-renewal ability of control and ZNF207 OE cells in E7 media with 20 ng/ml bFGF. Scale bars represent 17.5 mm. The counts of colonies are shown on the right. Data are presented as the mean ± SEM and are derived from three independent experiments