Fig. 1

Dynamic distribution of uterine group 1 innate lymphoid cells (g1 ILCs) during reproductive life. a Gating strategy for analysis and sorting by flow cytometry, with all freshly isolated g1 ILCs at mid-gestation (gd 9.5) gated on scatter and then defined as single live CD45⁺CD3⁻CD19⁻CD11b^low/-NK1.1⁺NKp46⁺; ILC1 cells defined as CD49a⁺Eomes⁻; tissue-resident NK (trNK) cells defined as CD49a⁺Eomes⁺ and conventional NK (cNK) cells defined as CD49a⁻Eomes⁺. Each panel is representative of at least a hundred independent samples. b Landscape of uterine g1 ILCs during reproductive life; numbers in plot indicate mean percentage of each individual subset of g1 ILCs as gated within live CD45⁺CD3⁻CD19⁻CD11b^low/-NK1.1⁺NKp46⁺ parent population. The size of the mean data points (filled circles) is proportional to the increasing percentage of the indicated subset. Inset shows the same time-point as the dashed rectangle below but in non-breastfeeding females. Data are representative of fifteen independent experiments with three individual animals for each time-point analysed. Mating for experiments (just after animals turn 8-weeks old) and partum (gd 19.5–21.5) time-points are indicated by the arrows on the plot. w week, d day, gd gestation day, pp post-partum, BF breastfeeding, nBF non-breastfeeding