Fig. 2
From: Molecular definition of group 1 innate lymphoid cells in the mouse uterus
Genome-wide transcriptomes of uterine g1 ILCs at mid-gestation. a Unbiased principal component analysis (PCA)-based clustering of uterine ILC1s, trNK and cNK cells and of liver ILC1s and cNK cells. The 250 most variable genes and the two principal components were used for clustering and to describe the variance between the subsets. b Heat map of all significant differentially expressed genes with a log2fold change >7.5, selected from comparisons described in the text and presented in Fig. 3a. c Individual gene expression plots of genes encoding proteins used for sorting g1 ILCs and additional genes used for validation of the ILC and NK-lineages. Scale represents log2(FPKM + 1) transformed normalised reads. d Individual gene expression plots of genes encoding F4/80, MERTK, CD86 and B220 in uterine and liver g1 ILCs and flow cytometry validation of their protein products. Histograms show protein expression on three uterine g1 ILCs gated as gated in Fig. 1a (red line-ILC1s, blue line-trNK, orange line-cNK). FPKM fragments per kilobase of exon model per million reads mapped
