Fig. 4
From: Molecular definition of group 1 innate lymphoid cells in the mouse uterus
Uterine g1 ILCs express CXCR6, IL22 and some cells are ex-ILC3s. a Individual gene expression plot showing expression of Cxcr6 in the liver and uterus. Scale represents log2(FPKM + 1) transformed normalised reads. Histograms are showing CXCR6 protein expression on g1 ILCs in both organs at mid-gestation. Red line-ILC1, blue line-trNK, orange line-cNK. G1 ILCs were gated as described in Fig. 1a. b Fate-mapping of g1 ILCs in the uterus of Rorc(γt)-CreTG/R26R females. c IL-22 production by uterine trNK and cNK at gd 9.5. Control wells were incubated with medium containing protein transport inhibitors only. d Quantification of the percentage of IL-22-producing cells in the uterus at gd 9.5. Error bars represent mean ± SD. e Quantification of the mean fluorescence intensity of IL-22 on g1 ILCs at gd 9.5. Error bars represent mean ± SD. f Flow cytometry validation of various surface receptors on g1 ILCs in the uterus. Data are representative of three independent experiments. FPKM: fragments per kilobase of exon model per million reads mapped
