Fig. 6

Pathways dysregulated by genetic alterations. a Seven pathways were significantly dysregulated in three or more NASH-HCCs from HFHC-fed mice ( ≥ 3 genes involved in each sample, adjusted P < 0.05). b Mutated genes in calcium signaling pathway are indicated. 28 genes were mutated in NASH-HCCs, 6 of which were recurrently mutated. Only one gene in calcium signaling pathway was mutated in one of the steatosis-HCCs. Color-filled icons show mutated genes. Recurrently mutated genes were highlighted with affected NASH-HCC numbers denoted. c Mutated genes in insulin signaling are indicated. 16 gene were mutated in NASH-HCCs, 2 of which were recurrently mutated, while no gene was mutated in steatosis-HCCs. d Five pathways were enriched commonly by mutations and aberrant gene expression in NASH-HCCs