Fig. 2
Site-specific inflammation induced erosions appear in response to mechanical stress. a Lateral view of micro-CT scans of CIA under voluntary running (right panel), unloaded (left panel) or control conditions (middle panel). Displayed mice had a similar clinical arthritis score. Talus (1), calcaneus (2), cuboid bone (7), MTV (8), MTIV (9). Scale bar: 1 mm. b Total bone surface erosion, cumulative quantification of bones in the midfoot and hindfoot shows significant increase of erosion by biomechanical loading (cuneiform I (4), navicular bone (3), cuboid bone (7), calcaneus (2), sesamoid tarsal bone (0)). CIA in voluntary running, unloaded or control conditions n = 8–10, KW and post-test. c Surface bone erosion quantification in CIA of cuneiform I for voluntary running mice shows increase vs. controls, MWU, n = 8–10/group. d Bone erosion quantification in CIA of metatarsal V and metatarsal II under voluntary running or control conditions for mice with varying degrees of clinical arthritis scores at the scanned hind paw (range 1–4, n = 6–7). Voluntary running causes increased bone erosion at MTV but not at MTII, significant for range 2–4 MWU. e Plantar view of micro-CT of cuneiform I in CIA from voluntary running mice (white rectangle). f Micro-CT of MTV in CIA under voluntary running conditions (white rectangle), plantar side. Error bars show the mean ± s.e.m., data are pooled from two independent experiments *p < 0.05, ***p < 0.001
