Fig. 7
Increased osteoclast number in mechano-sensitive regions. TRAP staining of dorsal sections used for osteoclast count of the individual bones (minimal six sections, TNFΔARE Control (n = 9); TNFΔARE Voluntary running (n = 11) collected from two independent experiments. a Highest osteoclast numbers were noted at the CCM region, osteoclast number increases at this side with biomechanical stress (voluntary running compared to control condition). Significantly lower numbers of osteoclasts detectable in the enthesis-poor MTII vs. enthesis-rich MTV region; as well as parallel increase of osteoclast numbers with activity at the enthesis-rich MTV region, MWU. b The left panel shows in situ osteoclast localization at the peroneus groove; black rectangle = peroneus groove with peroneus longus (PL) at lateral side calcaneus. The right panel shows the (c) CM with localized osteoclast formation; black square encircles cuboid (plantar side) with peroneus longus running through the plantar groove where osteoclasts are localized, and the enthesis rich base of MTV. Scale bar: 0.25 mm. Error bars show the mean ± s.e.m., *p < 0.05, **p < 0.01, ***p < 0.001
