Fig. 4

CaMPARI2 activity and PC in mouse primary visual cortex. a Schematic of the experimental setup (left). Two-photon fluorescence signal from cortical layer 2/3 neurons in V1 after PC (right). b Changes in fluorescence signal from four example cells marked in a. Cell numbers 1–2 are PC-tuned and have high OSI (0.8 and 0.92, respectively), cell number 3 is responsive and broadly tuned (OSI = 0.22), and cell number 4 is responsive and not PC-tuned (OSI = 0.96). c Correlation between peak ΔF/F₀ for the northward moving grating stimulus and the red-to-green ratio for individual responsive cells expressing CaMPARI2. Cells were grouped into four categories: non-responsive (not significantly responsive cells, gray dots), broadly tuned (significantly responsive and OSI < 0.5, cyan), PC-tuned (OSI > 0.5 and significant response to northward moving grating stimulus, magenta), and not PC-tuned (OSI > 0.5 and no significant response to northward moving grating stimulus, green). d Comparison of red-to-green ratio distribution of the four groups mentioned in c. PC efficiency was higher for PC-tuned cells, leading to a significant increase in red-to-green ratio. *p < 0.05, ***p < 0.001 (Wilcoxon Rank-Sum Test). Error bars indicate the standard error. Scale bar is 40 µm