Fig. 5
From: Improved methods for marking active neuron populations
Anti-CaMPARI-red antibody. a Schematic representation of the protocol used to generate the anti-CaMPARI-red monoclonal antibody. b Green (top) and red (bottom) endogenous CaMPARI2 fluorescence (left and middle columns) in mouse brain tissue imaged before (left column) and after (middle column) chemical fixation with paraformaldehyde. The right column shows the same region of fixed tissue after antibody staining with anti-FLAG antibody for total CaMPARI (top, cyan) and anti-CaMPARI-red antibody (bottom, orange). Note that following chemical fixation much of the red CaMPARI fluorescence was lost but was recovered with the anti-CaMPARI-red antibody staining. Scale bar is 50 µm
