Fig. 4
From: Structural basis of Gip1 for cytosolic sequestration of G protein in wide-range chemotaxis
Subcellular localization of endogenous Gip1 and Gβ. a Preferential complex formation between G proteins and Gip1. Cells were fractionated, and each fraction was used for the IP of F2G or F2G-tagged Gγ. The indicated proteins were visualized by immunoblotting using anti-Gip1, Gβ, and Flag antibodies. b The amount of endogenous Gβ and Gip1. Cells were fractionated to obtain whole-cell extract (W), supernatant (S), and precipitant (P), and their containing proteins were estimated in comparison to purified protein standards. The bands indicated by arrows represent His-Gβ, Gβ, and Gip1. Bands with an asterisk denote nonspecific bands
