Fig. 4
From: Transition path times of coupled folding and binding reveal the formation of an encounter complex
Ionic-strength dependence of ACTR-NCBD binding. a Association (kon) and b dissociation rate coefficients (koff) as a function of ionic strength from measurements at different NaCl concentrations. Error bars indicate standard errors obtained from 100 bootstrapping trials (sample sizes are listed in Supplementary Table 1). c Average transition path time, 〈tTP〉, and d transfer efficiency in the encounter complex, \(\hat E_{\sf {I}}\), as a function of ionic strength. Error bars indicate standard errors from 1000 bootstrapping trials (sample sizes are listed in Supplementary Table 3). e Schematic of the coupled folding and binding process with free-energy surfaces in the absence (solid line) and presence (dashed line) of ~0.4 M salt. Cartoon based on PDB entries 1KBH15 and 2KKJ17
