Fig. 1
Progressive changes in neocortical interneuron output of dividing NKX2.1+ MGE/PoA RGPs. a Images of the somatosensory cortex of representative P21 mouse brains that received in utero intraventricular injection of high-titer EGFP-expressing retrovirus at E12-E17. EGFP-labeled interneurons are shown in black. L, layer; WM, white matter. Scale bar: 150 μm. b 3D reconstruction images of P21 brains that received in utero retrovirus injection at different embryonic stages. Blue lines indicate the contours of the whole brain. Red and blue dots represent the cell bodies of the EGFP-expressing interneurons in the superficial (L2-4) and deep (L5/6) layers of the neocortex, respectively. A anterior, P posterior, D dorsal; V ventral. c Quantification of the total number of EGFP-expressing interneurons per hemisphere of the neocortex. (n = 6 hemispheres per time point from at least three different animals). Center line, median; box, interquartile range; whiskers, minimum and maximum. Red dots indicate individual data points. ****P < 0.0001 (P = 3.4e-13, Jonckheere–Terpstra test). d Quantification of the laminar distribution of EGFP-expressing interneurons. Data are presented as mean ± SEM (n = 6 hemispheres per time point from at least three different animals). *** P = 0.0001; **** P < 0.0001 (Kruskal-Wallis test)
