Fig. 7

Coregulator binding preferences of the unique and mutual T0070907-bound conformations. a Snapshot overlay of 2D [¹H,¹⁵N]-TROSY-HSQC spectra of T0070907-bound ¹⁵N-labeled PPARγ LBD titrated with NCoR1 peptide. b Extracted 1D planes of the NCoR1 spectra shown in a show the qualitative binding trends observed by NMR, whereas c an overlay of the extracted 1D planes is quantitatively described by d spectra using a 4-state model where the two slowly exchanging receptor populations (R and R*) are capable of binding to the peptide (RP and R*P), and receptor isomerizes in both the free and peptide-bound states (R ↔ R*, and RP ↔ R*P). (e–h) Analysis of performed for T0070907-bound ¹⁵N-labeled PPARγ LBD titrated with TRAP220 peptide performed similarly to the NCoR1 analysis described in a–d, which is also quantitatively described using the same 4-state model