Fig. 8
From: A structural mechanism for directing corepressor-selective inverse agonism of PPARγ
Coregulator binding preferences of the single GW9662-bound conformation detected by NMR. a Snapshot overlay of 2D [1H,15N]-TROSY-HSQC spectra of GW9662-bound 15N-labeled PPARγ LBD titrated with NCoR1 peptide. b The 15N spectral planes of the 2D NMR titration are quantitatively described using a 3-state model (R + P ↔ RP ↔ R*P) where PPARγ LBD bound to GW9662 and NCoR1 slowly isomerizes between two states (RP and R*P). c Snapshot overlay of 2D [1H,15N]-TROSY-HSQC spectra of GW9662-bound 15N-labeled PPARγ LBD titrated with TRAP220 peptide. d The 15N spectral planes of the 2D NMR titration are quantitatively described using a 2-state model (R + P ↔ RP)
