Fig. 1

Shh signaling is necessary for HFN following injury. a X-gal staining of large wound (LW) and small wound (SW) from of Gli1-LacZ mice at indicated time (n = 2–4 W (2–5 M) per condition). b qRT-PCR for Shh expression in SW and LW of wild-type mice at 7 days after complete re-epithelialization (n = 2–6 W (2 M) per condition). c Immunohistochemistry of Shh on SW and LW of wild-type mice at 7 days after complete re-epithelialization. *Indicates non-specific signals. d–f K14-CreER; Shh ^fl/fl (K14- Shh ^fl/fl) and littermate control mice were subjected to large wound (LW) and treated with TAM from PW3d until tissue harvest at PW21d (n = 11–12 W (11–12 M) per condition). Whole-mount HFN assay (d) and quantifications (e, f). g–j Pdgfra-CreER; Smo ^fl/fl (Pdgfra-Smo ^fl/fl) and littermate controls were subjected to large wound (LW) and were treated with TAM from 3 or 4 weeks before wounding until tissue harvest at PW21d (n = 3–5 W (3–5 M) per condition). Whole-mount HFN assay (g, i) and quantifications (h, j). n: number of wounds (W) or mice (M), Data are represented as mean ± s.d., *p < 0.05; **p < 0.01; ***p < 0.001; Student’s t-test, Dashed white circle: wound boundary, dashed line: epidermis–dermis border, DP dermal papilla, AP alkaline phosphatase, FE follicular epithelium, PW post-wound, Scale bars represent 500 µm (a (whole mount), d, g, i), 50 µm (a (section), c)