Fig. 3

Dermal Hh activation is sufficient to promote HFN in non-regenerative wounds. a Detection of tomato reporter on SW of SM22-rtTA; tetO-Cre; R26-Tomato mice at indicated time. b–n SM22-rtTA; tetO-Cre: R26-SmoM2 (SM22-SmoM2) and littermate controls were subjected to SW and treated with doxycycline from PW1d until tissue harvest at PW30d (n = 18 W (4–5 M) per condition). Whole-mount HFN assay (b, d) and quantifications (c, e). Percentage of AP⁺ DP with K17⁺ FE (f). Percentage of K17⁺ HF with hair shaft (HS) (g). Immunohistochemistry with indicated markers (h–m) and H&E (n). n: number of wounds (W) or mice (M), Data are represented as mean ± s.d., *p < 0.05; Student’s t-test, Dashed white circle: wound boundary, Dashed line: epidermis–dermis border, SW small wound, PW post-wound, DP dermal papilla, AP alkaline phosphatase, FE follicular epithelium, HF hair follicle, HS hair shaft, Bu bulge stem cell area, SG sebaceous gland. Scale bars represent 500 µm (b, d), 100 µm (n (Control)), 10 µm (a, h–m, n (SM22-SmoM2))