Fig. 4

Dermal Hh activation induces DP fate in wound fibroblasts. scRNA-seq was performed with Tomato⁺ cells isolated from wound dermis of both SM22-rtTA; tetO-Cre; R26-SmoM2/Tomato (SM22-SmoM2) and SM22-rtTA; tetO-Cre; R26-Tomato (control) mice 3 days after complete re-epithelialization. The mice were subject to SW and treated with doxycycline from PW1d until PW12d (n = 12–25 W (3–5 M) per condition). a–i tSNE plots of 4680 SM22 + dermal cells split between control and Hh activated conditions. tSNE plot of SM22⁺ dermal cells colored by assigned lineages (a). tSNE plot of SM22⁺ dermal cells according to lineage-specific markers (b–f). tSNE plot of SM22⁺ dermal cells according to Hh pathway components (g). tSNE plot of SM22⁺ myofibroblasts according to Hh pathway components (h). tSNE plot of SM22⁺ myofibroblasts according to cell origin (i). j Heatmap showing the expression of DP signature genes. Yellow and black/purple correspond to high and low expression levels, respectively