Fig. 5

Dermal Wnt activation alone is not sufficient for HFN. a Violin plots of Wnt pathway-related genes in SM22⁺ myofibroblasts. b X-gal staining in epidermis and dermis of SW of Axin2-LacZ mice at PW10d and PW32d. c–h SM22-rtTA; tetO-Cre; β-catenin ^fl(ex3)/+ (SM22-ex3) and littermate controls were subjected to SW and treated with doxycycline from PW1d until tissue harvest at PW30d (n = 3–4 W (3–4 M) per condition). Whole-mount HFN assay (c) and quantifications (d, e). H&E (f, g) and AP/Lef1 staining (h) show lack of hair germ (HG) formation by β-catenin activation in dermis. i–l SM22-rtTA; tetO-Cre; Wls ^fl/fl (SM22-Wls ^fl/fl) and littermate controls were subjected to LW and treated with doxycycline from PW3d until tissue harvest at PW21d (n = 4–5 W (4–5 M) per condition). Whole-mount HFN assay (i, k) and quantifications (j, l). n: number of wounds (W) or mice (M), Dashed white circle and zigzag line: wound boundary, Dashed line: epidermis–dermis border, SW small wound, LW large wound, AP alkaline phosphatase, PW post-wound, Scale bars represent 500 µm (b, c, i, k), 100 µm (f), 50 µm (g, h)