Fig. 2

Obesogenic diet feeding changes the fecal microbiota, which precedes glucose intolerance in mice. Mouse fecal samples were taken over the first 7 days (D0–D7) of feeding obesogenic, low fiber, high fat diets (HFD) and processed for bacterial DNA sequencing (N = 7–8). All mice were on a Chow diet on Day 0 (D0). a PCoA of Bray-Curtis dissimilarity for all samples over the 7 days on Chow diet, 45% HFD, or 60% HFD. b PCoA of Bray-Curtis dissimilarity after 3 days of eating each of the 3 diets. c Stacked bar graph showing the relative abundance of the 12 most abundant bacterial taxa (Genus level) over the first 7 days of eating each of the 3 diets. d Heat map of the 36 microbial taxa that were significantly different 3 days after eating Chow, 45% HFD, and 60% HFD. Non-parametric analysis of variance for each taxon between groups was conducted using the Kruskal–Wallis test. Taxa that passed the significance threshold of p < 0.05 were analyzed using the pairwise Wilcoxon rank sum test. Correction for multiple hypothesis testing (FDR) was calculated using the Benjamini-Hochberg method. Statistical significance was accepted at p < 0.05.  Fold change in relative abundance of the taxa that significantly changed between the groups was expressed relative to Chow and plotted in the heatmap