Fig. 3

Rapid changes in the composition of the fecal microbiota are maintained during prolonged obesogenic diet feeding. Fecal samples were taken 3 days (D3) and at 14 weeks (W14) after feeding chow or each obesogenic diet and processed for bacterial DNA sequencing (Chow = 12, 45% HFD = 13, 60% HFD = 12 mice). a PCoA of Bray-Curtis dissimilarity between each of the 3 diet groups (Day 3 and Week 14) (top panel), between the two obesogenic diet groups only (Day 3 and Week 14) (middle panel), and between Day 3 and Week 14 of mice that only ate the chow diet (bottom panel). b Stacked bar graph showing the relative abundance of the 12 most abundant bacterial taxa (Genus level) at Day 3 and Week 14 mice fed each diet. c Heat map of the 69 microbial taxa that differed between mice fed Chow, 45% HFD, and 60% HFD on Day 3 or Week 14. The average relative abundances of each taxon detected in mice fed the respective diets for 3 days or 14 weeks were compared between groups. For each time point (Day 3 or Week 14), non-parametric analysis of variance for each taxon between the diet groups was conducted using the Kruskal–Wallis test. Taxa that passed the significance threshold of p < 0.05 were analyzed using the pairwise Wilcoxon rank sum test. Correction for multiple hypothesis testing (FDR) was calculated using the Benjamini-Hochberg method. Statistical significance was accepted at p < 0.05.  Fold change in relative abundance of the taxa that significantly changed between the groups was expressed relative to Chow within each timepoint and plotted in the heatmap