Table 4 Multivariate analysis of biomarkers before treatment

From: Plasma Tie2 is a tumor vascular response biomarker for VEGF inhibitors in metastatic colorectal cancer

 

Hazard ratio (95% CI)

p-value

Bootstrap (frequency of p < 0.05)

Performance status 1

1.50 (0.89–2.54)

0.13

35.9%

Performance status 2

4.96 (1.42–17.41)

0.012

67.3%

Total tumor volumea

1.10 (1.05–1.16)

0.0003

79.7%

VEGFR2:Ktrans b

1.01 (1.00–1.02)

0.0008

96.3%

  1. Biomarkers with univariate p-values < 0.05 (Table 3) were included in the subsequent multi-variate analysis. The results from multivariate analysis were validated using a bootstrap resampling method, which examines the stability of the findings through removal of different data points with re-evaluation of the remaining data. In the multivariate analysis all HRs exceeded 1.00 inferring that the higher the parameter/biomarker, the worse the outcome
  2. VEGFA and VEGFR2 were correlated circulating biomarkers but given the relationship between VEGFA and Ktrans, it was biologically more plausible that the signal reflects VEGFR2. To allow comparisons of more than one biomarker with PFS, VEGFR2, and Ktrans were analyzed as a ratio (VEGFR2:Ktrans)
  3. atotal tumor volume/100 calculated from CT scan
  4. bVEGFR2/Ktrans WTV and ETV calculated from MRI scan
  5. VEGFA and R2 vascular endothelial growth factor A and receptor 2, HR hazard ratio, Ktrans endothelial transfer constant, PFS progression-free survival, CI confidence interval, MRI magnetic resonance imaging, CT computerized tomography scan
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