Fig. 5

Proliferation of AF-1⁰, AF-2⁰ and ERα^−/− cells in chimeric epithelia during pregnancy. a Representative fluorescence stereomicrographs of chimeric epithelia from WT.GFP⁺ or ERα mutant.GFP⁺ and WT.DsRed⁺ cells mixed in a 1:10 ratio. Hosts were analysed at P16–18. Scale bars; 1 mm. b Pie charts showing the number of mammary glands presenting only DsRed⁺ epithelial regenerations (red) or mixed ⁺ and GFP⁺ regenerations (red and green stripes) based on evaluation on “low magnification 7.8×” fluorescence stereomicrographs; from top to bottom, n = 20, 7, 7 and 6. c Dot plot showing percentage of area filled with GFP⁺ structures over total area filled in chimeric glands during pregnancy; evaluation at 7.8× magnification; shown are means ± SEM. d Dot plot showing ratios of GFP over DsRed signal intensity of different chimeras in pregnant hosts (n = 4–16; black line: median)