Fig. 7

Global gene expression profile of ERα^−/− and WT MECs. a Principal component (PC) analysis of RNAseq data showing the importance of the Esr1 genotype for global gene expression. b Bar graph showing the number of genes whose expression increased (IE) or decreased (DE) in ERα^−/−.GFP⁺ vs. WT.GFP⁺ MECs engrafted intraductally to contralateral glands of NSG females and subsequently isolated by FACS-sorting from hosts during pregnancy. c Bar plot showing reads per kilobase million (RPKM) of ERα high, sensor cell markers expressed in ERα^−/−.GFP⁺ and WT.GFP⁺ MECs that were engrafted intraductally to contralateral glands of NSG hosts and subsequently isolated by FACS-sorting from pregnant hosts. d Bar plot showing RPKM of milk genes expressed in FACS-sorted ERα^−/−.GFP⁺ and WT.GFP⁺ MECs grafted intraductally and isolated from pregnant hosts. e, f MetaCore analysis of genes showing decreased (e) or increased (f) expression, in ERα^−/−.GFP⁺ MECs compared to WT.GFP⁺ MECs. The most significantly enriched terms are listed with p value. g, h Reactome analysis of genes with decreased g or increased h expression, in ERα^−/−.GFP⁺ MECs compared to WT.GFP⁺ MECs. The most significantly enriched terms are listed with p value. i Visual representation of GO terms with increased expression in ERα^−/−.GFP+ vs. WT.GFP+ MECs by Cytoscape. j Pie chart showing proportions of KEGG terms among the genes with increased expression in the ERα^−/−.GFP⁺ vs. WT.GFP⁺ MECs