Fig. 3
From: Dual HLA B*42 and B*81-reactive T cell receptors recognize more diverse HIV-1 Gag escape variants
Molecular analysis of TCR β clonotypes in mono- and dual-reactive T cells. TCR β sequencing was performed on single FACS-sorted mono- and dual-TL9 tetramer reactive T cells from three B*81:01 expressing participants (a) and three B*42:01 expressing participants (b). TRBV and CDR3 sequences were determined using the IMGT V-quest tool (www.imgt.org). The total number of sequences collected per population is indicated under each pie chart. Unique TCR β clones are displayed as wedges in the pie chart. The size of the wedge indicates the frequency of each sequence within the population and the color represents TRBV usage. TCR β sequences in mono- and dual-reactive populations from B*81:01 expressing individuals were highly enriched for TRBV12-3/12-4 usage (indicated in gray); however, no public sequences were observed among these individuals. Mono-reactive populations from B*42:01 expressing individuals encoded diverse TRBV and also lacked public sequences. In contrast, dual-reactive populations from B*42:01 expressing individuals were enriched for TRBV12-3/12-4 usage (gray), and these sequences were comprised predominately of four identical (public) TCR β clones (highlighted by colored boxes). Notably, these public clones were distinct from any TCR observed in B*81:01 individuals
