Fig. 4
From: Dual HLA B*42 and B*81-reactive T cell receptors recognize more diverse HIV-1 Gag escape variants
In vitro validation of TCR specificity and dual-reactivity. Details for the eight TL9-specific TCR clones investigated in this study are shown, including donor HLA, mono- or dual-reactivity phenotype, paired TCR α/β V gene usage and CDR3 sequences (a). Jurkat T cells were co-transfected with TCR α/β, CD8 α and an NFAT-driven luciferase reporter vector, and then co-cultured with TL9 peptide-pulsed (b) or HIV-infected (c) target cells stably expressing B*81:01 or B*42:01. TCR-dependent NFAT signaling was quantified by luminescence. The expected mono- or dual-reactive phenotype was observed for all reconstructed TCR clones, as indicated by greater luminescence (absolute light units, y-axis) in the presence of TL9-pulsed or virus-infected target cells compared to no-peptide or uninfected controls. Assays were conducted at least three times. Results from a representative experiment are shown as the mean of three co-culture reactions, plus standard deviation
