Fig. 3
Practical application of Methylscape approach. a Differential affinity/adsorption of genomic DNAs as a function of their cancer and normal origin. Bars represent the relative current (%ir) mean values for DNA genomes derived from various normal (Black bars: Human Mammalian Epithelial Cell (HMEC), Prostate Epithelial cell (PrEC)) and cancer cell lines (Red bars: Breast cancer: BT474, MCF7, T47D, LNCap prostate cancer, HCT116 colorectal cancer and H-1975 lung cancer). Each data point represents the average of three separate trials, and error bars represent the standard deviation of measurements (%RSD = < 5% for n = 3). Right panel: Corresponding DPV graphs for all cell line DNAs and their respective baseline. b Individual box plot showing mean relative current (%ir) values generated by electrochemical detection of genomic DNAs extracted from normal and cancer tissues of various cancer types (breast, prostate and lymph node). c The combined data for all the tissue samples derived from 31 healthy individuals and 72 cancer patients, Right: the ROC analysis and diagnostic test evaluation shows the Disease Prevalence (DP), positive predictive values (PPV), negative predictive values (NPV) and accuracy of the method. d Box plot showing relative current (%ir) mean values generated by electrochemical detection for genomic DNAs extracted from plasma samples derived from 45 healthy individuals and 100 breast cancer patients, Right: the ROC analysis and diagnostic test evaluation shows the Disease Prevalence (DP), positive predictive values (PPV), negative predictive values (NPV) and accuracy of the method. In the box and whisker plots, the middle lines of the boxes represent the median (50th percentile) and the terminal line of the boxes represents the 25th to 75th percentile. The whiskers represent the lowest and the highest value
