Fig. 2
From: Transcriptome-wide identification of transient RNA G-quadruplexes in human cells
Isolation of G4 targets from human cell extracts using G4RP. (left) Schematic representation of G4RP protocol. G4RP signals of biotin control versus BioTASQ through RT-qPCR quantification of a VEGFA and b NRAS mRNA levels in untreated MCF7 cells. c Changes induced by BRACO-19 and RHPS4 measured by the fold change of G4RP signal in VEGFA, NRAS, TERF2, and HPRT1 mRNA. Values are normalized to their individual untreated controls. Three biological replicates were used for the quantifications. Student's t-test and two-way ANOVA were performed. p-Values: *p < 0.05, ** p < 0.01, and ***p < 0.001. Error bars represent SEM
