Fig. 2 | Nature Communications

Fig. 2

From: Longitudinal single-cell RNA sequencing of patient-derived primary cells reveals drug-induced infidelity in stem cell hierarchy

Fig. 2

Single cell RNA-seq of naive, drug-resistant and drug-holiday cells from OSCCs. a Schematic representation of the generation of drug-resistant models and scRNA-seq workflow. b RaceID for Hierarchical k-means clustering of 1302 scRNA-seq libraries based on gene expression profiling identifies five major clusters. c t-SNE visualization of clusters identified from OSCC-PDPC scRNA-seq libraries. d Color-coding of t-SNE plot based on the identities of individual OSCC-PDPCs and their drug-resistant/holiday models. e Relative expression of genes associated with basal (KRT5/KRT14), luminal (KRT8/KRT18), epithelial (EPCAM/Ecad), and mesenchymal (VIM/SPARC) markers on tSNE plot suggesting that the clusters represent distinch phenotypic cell-states

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