Fig. 8 | Nature Communications

Fig. 8

From: Cell metabolism regulates integrin mechanosensing via an SLC3A2-dependent sphingolipid biosynthesis pathway

Fig. 8

Loss of CD98hc affects GEF-H1 recruitment in membrane microdomains. a, b Loss of CD98hc impairs recruitment of src kinases (a) and GEF-H1 (b) in CEMM. One experiment representative of n = 2. c GEF-H1 and cholesterol colocalize in control cells (arrows). Endogenous GEF-H1 and cholesterol were stained using an anti-GEF-H1 antibody or filipin III respectively. Right panel is a magnified crop of the white squared box. Scale bar is 50 µm. d Cholesterol depletion induces accumulation of GEF-H1 in membranes. e C12SM exogenous supply induces a decrease in the amount of GEF-H1 constitutively recruited to membrane fractions in CD98hc-null cells to levels similar to that of control cells

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