Fig. 6

Cas9–Cas9 fusions achieve robust and specific genome editing. a Summary of the GUIDE-seq genome-wide off-target analysis of SpCas9^WT, Sa/NmCas9^WT, and SpCas9^WT-Sa/NmCas9^WT at four GATA1 target sites (Supplementary Data 2). b Deep sequencing determined lesion rates for these nucleases at a subset of off-target sites discovered by the GUIDE-seq data or predicted by CasOFFinder (Supplementary Data 3). The names of SpCas9, NmCas9, and SaCas9 off-target sites are colored in dark red, blue, and green. The GUIDE-seq result is from single experiment, and amplicon deep sequencing data are from three independent biological replicates performed on different days in HEK293T cells (Supplementary Data 1). Error bars indicate ± s.e.m. Statistical significance is determined by one-way analysis of variance (ANOVA), *, **, ***, and NS denote BH-adjusted P-values of <0.05, <0.01, <0.001, and not significant, respectively