Fig. 1

Correlation between Ash1L expression and myoblast fusion. a Ash1L expression during muscle development. RT-qPCR analysis on muscle tissue from hindlimbs of mice from the embryonic stage E16.5 to adulthood (p28). Expression analysis of Myomaker, as a positive fusion gene, and adult myosin (Myh4) as a positive control for adult stages. Unpaired two-tailed t test. Confidence intervals 95%. n = 6. b Ash1L expression in regenerating muscle tissue. RT-qPCR analysis of Ash1L expression in tibialis anterior of wild-type adult mice, untreated (UNT), or 5 and 10 days after cardiotoxin (CTX) injection (left panel). Immunofluorescence for Ash1L (in green) and nuclear staining (Hoechst), in transverse cryosections from the tibialis anterior muscles of injured wild-type mice, 5 days after cardiotoxin injection (CTX 5 days) compared to untreated controls (Unt). Scale bar, 50 μm. Magnification ×65. Arrows indicate the Ash1L-positive nuclei. Unpaired two-tailed t test. Confidence intervals 95%. n = 4. c Ash1L protein level during in vitro muscle differentiation. Immunoblot of C2C12 cells at days 0 and 1, and densitometric analysis of Ash1L signal relative to vinculin as housekeeping protein (left panel). Immunofluorescence for Ash1L (in green) and nuclear staining (Hoechst), in C2C12 cells at days 0 and 1 of in vitro muscle differentiation. Scale bar, 100 μm. Magnification ×20. Paired two-tailed t test. Confidence intervals 95%. Data are the mean for three independent experiments. d Ash1L protein level in proliferating myoblasts vs. confluent cells. Comparison between proliferating myoblasts (P) and confluent cells (C). Paired two-tailed t test. Confidence intervals 95%. Data are the mean for three independent experiments. Source data are provided as a Source Data file. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001. NS not significant