Fig. 2
From: The Trithorax protein Ash1L promotes myoblast fusion by activating Cdon expression
Ash1L GT mice display muscle hypoplasia. a Gomori-trichrome staining of transverse cryosections from wild-type (WT) and Ash1L GT mice, at embryonic stage E18.5 (upper panel). Quantification of: the total number of myofibers and myofibers cross-sectional area (CSA) (lower panel). n = 3. Scale bar, 100 µm, magnification ×20. Unpaired two-tailed t test. Confidence intervals 95%. Results come from six biological replicates. b Immunofluorescence for laminin (in green) and nuclear staining (Hoechst) in longitudinal cryosections from wild-type (WT) and Ash1L GT mice, at the embryonic stage of E18.5 (left panel). Quantification of the number of myonuclei normalized on myofiber length in mm (myonuclei/myofibers) (right panel). Scale bar, 100 µm, magnification ×20. Unpaired two-tailed t test. Confidence intervals 95%. Results come from seven biological replicates. Source data are provided as a Source Data file. *p ≤ 0.05
