Fig. 8

Cdon expression rescues fusion defects caused by Ash1L knockdown. a Immunofluorescence for myosin heavy chain and nuclear staining in C2C12 cells treated with two consecutive rounds of transient transfections: non-targeting (NS) or Ash1L siRNAs (Ash1L KD), and empty vector (EV) or Cdon-expressing vector (Cdon OE), before inducing differentiation for 3 days (left). Scale bar, 100 µm. b RT-qPCR analysis of Ash1L and Cdon expression in C2C12 cells transfected with: empty vector plus non-targeting siRNAs (EV + NS), empty vector plus Ash1L siRNAs (EV + Ash1L KD), Cdon-expressing vector plus non-targeting siRNAs (Cdon OE + NS), and Cdon-expressing vector +Ash1L siRNAs (Cdon OE + Ash1L KD). c Fusion index and nuclei distribution for each condition were calculated as described for Fig. 3. Unpaired two-tailed t test. Confidence intervals 95%. n = 3. Source data are provided as a Source Data file. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. NS not significant