Fig. 1
From: Targeting fidelity of adenine and cytosine base editors in mouse embryos
Base editing frequency and accuracy. a Diagram of a generic target locus with different categories of editing outcomes. On-target (dark green): the target nucleotide within the 5-bp editing window; bystander (light green): a non-target nucleotide within the protospacer; proximal off-target (yellow): outside the protospacer but within 200 bp of the target site. b Frequency of alleles carrying on-target mutation, bystander mutation, proximal off-target mutation, and indels. Note that a single allele can carry more than one mutation. The total number of mutant alleles obtained with each base editor was set as 100% (HF2-BE2, 58; BE3, 96; SaBE3, 45; VQR-BE3, 27; BE4, 33; and ABE, 263). The frequency of each category is shown and number of edited alleles for each group and each base editor are shown in Table 1. c Frequency of alleles carrying unanticipated products (any mutation different from anticipated products: C•G-to-T•A for CBE, and A•T-to-G•C for ABE). d Frequency of alleles that carry only intended (anticipated) edits within the on-target region
