Fig. 2
From: A CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection
Schematic representation and fluorescent EMSA verification of CRISPR–Cas9-triggered linear SDA. a Schematic illustration showing binding of Cy3-labeled IP primer (green) to the exposed region of the non-target strand in the Cy5-labeled DNA–Cas9 ribonucleoprotein complex (red). b Fluorescent EMSA (6% PAGE) revealing the formation of DNA–Cas9 ribonucleoprotein complex (Species I) and IP–DNA–Cas9 complex (Species II). c Schematic illustration showing initiation of linear SDA from the IP primer after adding the SDA mixtures. d Fluorescent EMSA (6% PAGE) confirming successful strand elongation from the 3′ of IPT1-DNS-Cy3 primer to the upstream end of the pTF1-Cy5 fragment. * The slow migrating complex represents the DNA–RNA hybrid formed between IPT1-DNS-Cy3 and partially unfolded sgpTF1-DNS, whereas the fast migrating complex represents the hybrid between IPT1-DNS-Cy3 and fully folded sgpTF1-DNS. Uncropped gels are shown in Supplementary Fig. 14
