Fig. 3

CRISDA-based DNA amplification and detection with attomolar sensitivity. a Schematic of CRISDA-based DNA amplification and detection towards a 269 bp model DNA fragment pTF1. b Representative gel and endpoint fluorescence measurements illustrating that CRISDA is capable of highly sensitive amplification and detection of the DNA fragment pTF1. The arrow indicates successful amplification of a 186 bp amplicon. c Representative endpoint fluorescence measurements showing that CRISDA is capable of highly sensitive amplification and detection of a 169 bp (T1) and 203 bp (T2) region in the hTF1 DNA fragment derived from Chromosome 9 in the human genome. d Representative endpoint fluorescence measurements showing that CRISDA is capable of highly sensitive and specific amplification and detection of the T1 and T2 region in human genomic DNA samples. In each replicate, fluorescence intensities of CRISDA reactions with various target concentrations were normalized against the one containing the highest target concentration. n = 4 technical replicates, two-tailed Student’s t test; **P < 0.01, ***P < 0.001, ****P < 0.0001, bars represent mean ± s.d. Uncropped gels are shown in Supplementary Fig. 14