Fig. 7
Integrative analysis of phosphoproteomic and mRNASeq data. a Hierarchical clustering of correlation matrix for phosphoproteomic data reveals that most sites are correlated. Sub-clusters highlight known interactions as potentially novel signaling network. b Clustering of mRNA correlation matrix highlights the bi-modal response to Erlotinib, with most transcripts either decreasing or increasing on Erlotinib treatment and thus either positively or negatively correlated with other transcripts. c Clustering of phosphoproteomic and mRNASeq correlation matrix. Phosphorylation sites tend to decrease on Erlotinib treatement and are therefore positively correlated with approximately half of the transcripts and negatively correlated with the other half of the transcripts. Sub-clusters of phosphorylation sites indicate well-established signaling networks. d Line graphs showing analysis of correlation between phosphorylation sites and their transcripts indicating that activation/inhibition of the kinase may be regulating the site. Error bars represent the standard deviation
