Fig. 4
Similar ecologies from different FM donors elicit same beneficial effects. a, b Colon length (a) and cumulative histological score (b) of DSS (black boxes), DSS + IEO-FMT (white boxes), DSS + CR-FMT (dotted boxes) and DSS + Envigo-FMT (striped boxes) treated mice. c Colonic expression of Tnf and Il1b in DSS (black boxes), DSS + IEO-FMT (white boxes), DSS + CR-FMT (dotted boxes) and DSS + Envigo-FMT (striped boxes) treated mice d Comparison of relative abundancies of different taxa between Envigo- (outer chart), Charles River- (CR) (middle chart) or IEO-derived faecal microbiota. e Partial Least square-discrimination analysis (PLSD-DA) on metabolomics data between Envigo- (blue), Charles River- (CR, green) or IEO (red)-derived faecal microbiota. f Comparison of relative abundancies of different taxa between normobiotic-, IEO-, CR- Envigo- and dysbiotic-derived fecal microbiota. Statistical significance was calculated using a Mann–Whitney test for comparison within two groups or Kruskal–Wallis test with Dunn’s multiple comparison correction within more than two groups. *P < 0.05, **P < 0.01, ***P < 0.001 were regarded as statistically significant. Outliers were detected with Grubb’s test. Non parametric distributions were represented as median + /- interquartile range. In Box and whiskers plots, centre line represents median; cross, represents mean; dots represent outliers. In (a–c) DSS n = 7, DSS + IEO-FMT n = 10, DSS + CR-FMT n = 10, DSS + Envigo-FMT n = 10. In (d, f) IEO-FMT n = 9, CR-FMT n = 10, Envigo-FMT n = 9, nFMT = 7, dFMT = 7. In (e) DSS n = 5, DSS + IEO-FMT n = 6, DSS + CR-FMT n = 5, DSS + Envigo-FMT n = 5
