Fig. 5

Therapeutic FMT modulates immune cell frequencies and phenotypes. a Representative dot plots (upper panels), frequencies and absolute numbers (lower panels) of colonic CD4⁺ T cells and iNKT cells in DSS-treated (black bars) and FMT-treated (white bars) mice 11 days after starting DSS administration. b Representative dot plots, (upper panels), frequencies and absolute numbers (lower panels) of Ki67-expressing colonic CD4⁺ T cells and iNKT cells in DSS-treated (black bars) and FMT-treated (white bars) mice. c Representative dot plots, (upper panels), frequencies and absolute numbers (lower panels) of Ki67 and CD107a- expressing colonic CD8⁺ T cells in DSS-treated (black bars) and FMT-treated (white bars) mice. d Representative dot plots and frequency of dendritic cells (CD45.2⁺CD3^-CD11c⁺), macrophages (CD45.2⁺CD3^-F4/80⁺CD11b⁻), B cells (CD45.2⁺CD3⁻CD19⁺), neutrophils (CD45.2⁺CD3⁻Ly6g⁺CD11b⁺) in DSS-treated (left panels) and FMT-treated (right panels) mice 11 days after starting DSS administration. e Absolute numbers of colonic dendritic cells, macrophages, B cells, neutrophils in DSS-treated (black boxes) and DSS + FMT-treated (white boxes). f Absolute numbers of MHC-II expressing colonic dendritic cells, macrophages, B cells and neutrophils in DSS (white boxes) and DSS + FMT-treated (black boxes) mice. g Representative histograms of MHC-II expression and cumulative Mean fluorescence intensity (MFI) on colonic dendritic cells, macrophages, B cells, neutrophils in DSS (black boxes) and DSS + FMT (white boxes) treated mice. Statistical significance was calculated using a Mann–Whitney test for comparison within two groups or Kruskal–Wallis test with Dunn’s multiple comparison correction within more than two groups. *P < 0.05, **P < 0.01, ***P < 0.001 were regarded as statistically significant. Outliers were detected with Grubb’s test. Non parametric distributions were represented as median + /- interquartile range. In Box and whiskers plots, centre line represents median; cross, represents mean; dots represent outliers. In (a–c) DSS n = 12, DSS + FMT n = 12. In (d–g) DSS n = 16, DSS + FMT n = 16