Fig. 7
IL-10 contributes to FMT beneficial therapeutic effects. a IL-10 colonic expression in DSS-(black boxes) and DSS + FMT-(white boxes) treated mice. b, c Representative histograms of IL-10 expression (b) and cumulative Mean fluorescence intensity (MFI) (c, left panel) and frequency (c, right panel) of IL-10-producing colonic professional APC (CD45+CD3−MHC-II+) in DSS (black boxes) and DSS + FMT (white boxes) treated mice. d Summary of cytokines secreted by colonic CD4+ T cells and iNKT cells in DSS-treated (black boxes) and FMT-treated (white boxes) mice 11 days after starting DSS administration. e Schematic representation of the experiment. f, g Colon length (f) and weight loss (g) of untreated mice (white dotted boxes/open circles), or receiving DSS (black boxes/black symbols), or DSS + FMT (white boxes/white square symbols) or DSS + αIL-10R (green boxes/green symbols), or DSS + FMT + αIL-10R (blue boxes and blue symbols) or mice receiving DSS + FMT + αIL-10R isotype antibody (red boxes/red symbols). h Colonic expression of Tnf, Ifnγ and IL1β in DSS (black boxes), DSS + FMT (white boxes), DSS + αIL-10R (blue boxes), DSS + FMT + αIL-10R- (green boxes) and DSS + FMT + αIL-10R-isotype-antibody-treated mice (red boxes). i Absolute numbers of KI67 expressing colonic CD4+ T cells (left panels) and iNKT cells (right panels) in DSS (black boxes), DSS + FMT (white boxes), DSS + αIL-10R (blue boxes), DSS + FMT + αIL-10R (green boxes) and DSS + FMT + αIL-10R isotype antibody (red boxes). Statistical significance was calculated using a Mann–Whitney test for comparison within two groups or Kruskal–Wallis test with Dunn’s multiple comparison correction within more than two groups. *P < 0.05, **P < 0.01, ***P < 0.001 were regarded as statistically significant. Outliers were detected with Grubb’s test. Non parametric distributions were represented as median + /- interquartile range. In Box and whiskers plots, centre line represents median; cross, represents mean; dots represent outliers. In (a) DSS n = 15,DSS + FMT n = 26. In (b–d) DSS n = 14,DSS + FMT n = 17. In (e–i) DSS, DSS + FMT n = 4; DSS + αIL-10R,DSS + FMT + αIL-10R n = 5, DSS + FMT + isotype n = 3
