Fig. 3
IL-1 signaling activates MyD88-TRAF3-IRF3-dependent type I IFN signaling in vitro and negatively regulates type I IFN cytokine production in vivo. a–c WT, Il1r1−/−, Myd88−/−, and Irf3−/− pDCs were stimulated with recombinant mouse IL-1β for indicated times, and RNA from pDCs was isolated and used for expression analysis of Ifna (a), Ifnb (b), and Il6 (c) by using qPCR. d–f Traf3wt/wt CD11c-cre and Traf3f/f CD11c-cre pDCs were stimulated with recombinant mouse IL-1β for indicated times, and RNA from cells was isolated and used for expression analysis of Ifna (d), Ifnb (e), and Il6 (f) by using qPCR. g, h WT, Aim2−/−, Nlrp3−/−, Casp1−/−, and Il1r1−/− mice were intraperitoneally infected with P. yoelii YM. Serum was collected at indicated times and subjected to ELISA analysis of IFN-α (g) and IFN-β (h). Data are representatives of three independent experiments with similar results and plotted as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 vs. corresponding control. NS, not significant, ND, not detected
