Fig. 4 | Nature Communications

Fig. 4

From: Signal peptide represses GluK1 surface and synaptic trafficking through binding to amino-terminal domain

Fig. 4

The GluK1 signal peptide interacts with the ATD. a Immunoblots of immunoprecipitates from transfected HEK 293T cells lysates. The transfected constructs are indicated above each lane. Recombinant protein HA-GFP was used as a control for HA-SPGluK1-GFP, which lacks the GluK1 signal sequence in mature protein. HA-GFP, ATDGluK1-FLAG, and ATDGluK2-FLAG were driven by GluK2 signal peptide to ensure they were synthesized in ER pathway. b Fluorescence polarization (FP) analyses of peptide-protein. FITC-labeled GluK1 signal peptide (100 nM) was titrated with increasing concentrations of indicated GST-fusion proteins, and changes in FP were monitored and the apparent Kd values (mean ± SEM) were determined from three experiments. N.D., not determined for GST-ATDGluA2

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