Fig. 3

IL-7Rα⁺ ILC1 migration to skin-draining LNs is dependent on CXCR3. a, b Representative density plots showing Ki67 (a) or 7-AAD (b) staining on ILN CD3⁻NK1.1⁺IL-7Rα⁺ cells from OXA-sensitized (a: 48 h; b: 72 h) WT B6 mice. Statistical analysis of results showing the percentages of Ki67⁺ (a) or 7-AAD⁺ (b) cells among ILN ILC1s. Data are representative of two independent experiments (n = 5 in each group). c Representative dot plots (left panel) showing CXCR3 and IL-7Rα expression on CD3⁻NK1.1⁺NKp46⁺ cells from OXA-sensitized (48 h) B6 mice. Statistical analysis of results (right panel) showing MFI of CXCR3 on cNK cells and ILC1s. Data are representative of two independent experiments (n = 5 in each experiment). d mRNA expression of Cxcl9 and Cxcl10 in the indicated tissues after OXA sensitization. Data are pooled from two independent experiments (n = 3 in each group). e Representative density plots (left panels) showing CD49a and IL-7Rα expression on ILN CD3⁻ cells from OXA-sensitized (48 h) WT or Cxcr3^−/− mice (left panels). Numbers in dot plots represent the percentages of ILN IL-7Rα⁺ ILC1s among CD45⁺ cells. Statistical analysis of results (right panels) showing the percentages and absolute numbers of ILN IL-7Rα⁺ ILC1s. Data are representative of two experiments (n = 5 in each group). f Ear swelling of WT and Cxcr3^−/− mice sensitized (days 0, 1) and challenged (day 4) with OXA. Data are representative of two experiments (n = 5 in each group). g Ear swelling of Rag1^−/− and Rag1^−/−Cxcr3^−/− mice sensitized (days 0, 1) and challenged (day 4) with OXA. Data are representative of two experiments (n = 5–6 in each group). Means ± SEM are shown. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, two-tailed unpaired Student’s t test