Fig. 5

LN memory IL-7Rα⁺ ILC1s selectively reside in the liver. a LN group 1 ILCs from OXA-sensitized (72 h) Rag1^−/− mice (CD45.2⁺) (2 × 10⁵ cells) were adoptively transferred into CD45.1 mice. Donor cells were analyzed 2 months later. Representative density plots are of cells gated on CD3⁻NK1.1⁺. Data are representative of two independent experiments (n = 2 in each experiment). b LN IL-7Rα⁻ cNK cells or IL-7Rα⁺ ILC1s from OXA-sensitized (72 h) Rag1^−/− mice (CD45.2⁺) (1 × 10⁵ cells) were adoptively transferred into CD45.1 mice. One month later, donor cells were analyzed in recipient livers. Representative density plots are of cells gated on CD3⁻NK1.1⁺. Data are representative of two independent experiments (n = 2 in each experiment). c Expression of CD127, CD49a, CXCR3, CD49b, T-bet, and Eomes by donor-derived ILC1s in recipient livers of b. Histograms are of cells gated on CD3⁻NK1.1⁺CD45.2⁺CD45.1⁻. Data are representative of two independent experiments (n = 2 in each experiment). d Expression of CXCR6 on donor-derived cells in recipient livers of a. Data are representative of two independent experiments (n = 2 in each experiment). e OXA-sensitized WT (CD45.1⁺) and Cxcr6^−/− (CD45.2⁺) LN group1 ILCs (2 × 10⁵ cells) were adoptively transferred into sub-lethally irradiated CD45.1⁺CD45.2⁺ mice; recipient mice were analyzed 1 month later. Representative density plots (left panels) are of liver group 1 ILCs (CD3⁻NK1.1⁺), with host cells (CD45.1⁺CD45.2⁺) excluded. Statistical analysis of results (right panel) showing the percentages of CD45.1⁺ (WT) and CD45.2⁺ (Cxcr6^−/−) cells among total donor-derived group 1 ILCs (n = 3 in each group). Means ± SEM are shown. ****P < 0.0001, two-tailed unpaired Student’s t test