Fig. 5

Metastases harbor numerous low-abundance seeding clones. a Number of seeding clones, defined as barcodes detected regardless of copy number, is plotted. Barcodes present in metastases were only counted if they were also detected in the corresponding MFP tumor. The mean number of barcodes in each sample is denoted above each bar. (two-tailed T-tests, *p = 2.65e−7, **p = 0.00025, ***p = 0.024; error bars are SEM; n = 4 MFP tumors, n = 21 lung, n = 12 liver, and n = 4 brain metastases). b The percent of seeding clones detected in each metastatic lesion relative to the total number of unique barcodes detected in its matched MFP tumor is shown. c The number of dominant barcodes in metastases of each organ was compared with the total number of seeding clones detected in each metastasis. d The percentage of seeding clones that are unique to each metastatic site (detected in the MFP tumor of that mouse, but not in any other metastasis of that mouse) is plotted