Fig. 3
The C-terminal half of Pol2 contains a catalytically dead polymerase fold that has lost its DNA-binding function. a C-Pol2 contains an inactive polymerase fold with jaws wide open. b Coulombic surface coloring of the C-Pol2 catalytically dead polymerase reveals a lack of positive charges in the vestiges of the DNA-binding groove, while the DNA-binding site in the related Pol alpha catalytic domain is positively charged. c The DNA-binding groove in C-Pol2 is occupied by the Pol2 zinc-finger appendix. d DNA-binding assay with various Pol epsilon variants. Only constructs containing the N-Pol2 catalytic domain or the histone-related proteins Dpb3-Dpb4 bind to DNA. We were unable to detect any DNA binding for C-Pol2 and Dpb2. On the right, quantification of the DNA-binding assays. Each experiment was repeated three times and error bars indicates standard deviation. Also refer to Supplementary Fig. 3
