Fig. 8 | Nature Communications

Fig. 8

From: Structure of DNA-CMG-Pol epsilon elucidates the roles of the non-catalytic polymerase modules in the eukaryotic replisome

Fig. 8

Proposed mechanism for the concomitant recruitment of GINS and Pol epsilon onto MCM. By interacting with Cdc45 on the N-terminal MCM face, GINS stabilizes the Mcm2-5-3 N-terminal interactions. C-Pol2 and Dpb2 in the Pol epsilon complex play a similar role, by stabilizing the Mcm2-5-3 AAA+ ATPase interactions. Given that GINS and Pol epsilon are recruited onto MCM as part of the same complex, concomitant biding of both factors might promote activating conformational changes in the helicase, eventually leading to origin activation

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